Lactose is the main sugar in milk and it is also present in dairy products. The lactose is hydrolyzed in the body through an enzyme called lactase, giving rise to glucose and galactose that are absorbed in the intestine. People with lactase deficiency in the intestinal mucosa (hypolactasia) do not hydrolyze lactose properly, producing the characteristic symptoms of lactose intolerance. In most humans, the ability to digest lactose decreases after the breastfeeding period by a gradual decrease in the expression of the LCT gene. Expression of the LCT gene is regulated by a DNA sequence located in the MCM6 gene. Some individuals have certain polymorphisms in this gene that maintain the production of intestinal lactase, allowing these people to maintain the ability to properly digest milk throughout their lives.
The Lactose Test consists of genetic analysis of the patient’s DNA to determine the presence of polymorphisms in the 2q21.3 locus in the MCM6 gene (MCM6) associated with the development of lactose intolerance in adulthood. The examination is performed through the amplification and subsequent sequencing of the fragments of interest. Alternatively to the respiratory test, the analysis is performed from a blood sample, avoiding direct exposure of the patient to substances.
• Indicated in the assessment of hypolactasia in adults with clinical symptoms of lactose intolerance: abdominal pain and distension, bloating, gases, and diarrhea.
• Clinical suspicion of lactose intolerance.
• Guaranteed from the experience of the Synlab group, the absolute European leader in laboratory diagnosis.
• It uses the Sanger sequencing method that guarantees 100% coverage of all bases reads of the DNA.
• Any pathogenic mutation or mutation of uncertain significance is confirmed by a new DNA extraction.
• Alternatively to the respiratory test, the analysis is performed from a blood sample, avoiding direct exposure of the patient to substances.
DOCUMENTATION – Available on Extranet for clients:
• Informed Consent.
• Clinical Questionnaire.
• Fasting is not necessary for the test.